fluorescence microtiter plate reader polarstar galaxy  (BMG Labtech)

Journal: Journal of Dental Sciences

Article Title: 3,4-methylenedioxymethamphetamine induces reactive oxygen species-mediated autophagy and thioredoxin-interactive protein/nucleotide-binding domain, leucine-rich containing family, pyrin domain-containing-3 inflammasome activation in dental pulp stem cells

doi: 10.1016/j.jds.2025.04.025

Figure Lengend Snippet: Effects of MDMA on ROS-TXNIP-NLRP3 inflammasome axis in DPSCs. DPSCs were treated with 0, 10, 100, 300, and 500 μM of MDMA for 24 h. The ROS formation was detected by DCFH-DA dye with an inverted fluorescence microscope (A), and the fluorescence intensity was measured by a microtiter plate reader (B). Protein expression of TXNIP, NLRP3, ASC, pro-caspase-1, cleaved caspase-1, pro-IL-1β, and IL-1β were analyzed by Western blot (C). Quantitative analysis of TXNIP (D), NLRP3 (E), cleaved caspase-1 (F), and IL-1β (G) were conducted. Results represent the mean ± SEM (n = 3), analyzed via one-way ANOVA followed by a Bonferroni post-hoc test. ∗P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 versus control. Abbreviation: MDMA - 3,4-methylenedioxymethamphetamine, ROS - reactive oxygen species, TXNIP - thioredoxin-interacting protein, NLRP3 - nucleotide-binding domain, leucine-rich–containing family, pyrin domain-containing 3, DPSCs - dental pulp stem cells, DCFH-DA - 2′,7′-dichlorofluorescein diacetate, ASC - apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain, IL - interleukin.

Article Snippet: Fluorescence quantitation was measured using a fluorescence microtiter plate reader (POLARstar Galaxy, BMG Labtech, Ortenberg, Germany) at 485 and 530 nm wavelengths.

Techniques: Fluorescence, Microscopy, Expressing, Western Blot, Control, Binding Assay